Independent lab–confirmed · Eagle Science-Based Solutions
Laboratory testing
Eagle Science-Based Solutions ran two complementary studies on the Layer 1 surface chemistry in 2022 — an efficacy study measuring reduction across a broad organism panel, and a dispersion study measuring how evenly the chemistry distributes when applied. Both are independently lab-confirmed.
Independently lab-confirmed
Efficacy panel — Protocol PR-21047
The chemistry was evaluated against a 10-organism panel on three surface types — stainless steel, plexiglass, and vinyl — at a 10-minute dwell, under a protocol harmonizing EN 17272 and USP<1072>. Every organism on every surface met the study's acceptance criteria. Lab conclusion: the chemistry exhibits bactericidal, fungicidal, and sporicidal activity when used as directed.
| Measure | Result |
|---|
| Organism panel | 10 organisms (incl. Staphylococcus aureus ATCC 6538) |
| Surfaces tested | Stainless steel · plexiglass · vinyl |
| Dwell time | 10 minutes |
| Log reduction range | 4.5 to >6.0 across all organisms / surfaces |
| Outcome | All met acceptance criteria |
Source · Eagle Science-Based Solutions · Protocol PR-21047 · 2022
Independently lab-confirmed
Dispersion & distribution — Protocol PR-21049
A dispersion study under EN 17272 measured how evenly the chemistry reaches a surface when applied. Against Staphylococcus aureus (ATCC 6538) on stainless steel at a 10-minute dwell, it achieved a ≥5-log reduction at every test zone, meeting the protocol's acceptance criteria.
| Zone | Log reduction |
|---|
| Zone 1 | 5.7 |
| Zone 2 | >6.0 |
| Zone 3 | >6.0 |
| Zone 4 | 5.7 |
| Challenge level | 7.3 × 10⁶ CFU / coupon |
Source · Eagle Science-Based Solutions · Protocol PR-21049 · testing 03/14/2022 · approved 03/25/2022
These are CFU-based microbiological challenge tests (not ATP), so log-reduction figures against named organisms are reported as the laboratory measured them.
Attributed · manufacturer GLP testing
Manufacturer GLP testing
GLP testing commissioned by the manufacturer evaluated the Layer 1 surface chemistry for bactericidal and virucidal efficacy at a 10-minute dwell, including activity against a coronavirus — reported as a 99.9% reduction in under three minutes.
Source · manufacturer-commissioned GLP testing · March 2021 · presented as an attributed third-party finding. Regulated marketing terms used in the manufacturer's own collateral (e.g. "hospital grade," "eradicates") are deliberately not carried onto this page.
Independent residual study
Residual bioburden over time
An independent residual study tracked surface bioburden by ATP (RLU) measurement over a three-week window under continuous heavy-touch conditions — surfaces in daily multi-user contact, with no re-cleaning during the study — using a Hygiena SystemSURE Plus ATP system.
Source · independent residual (Purifly) ATP study · presented as a bioburden measurement and a description of the study design — not as a fixed "protects for three weeks" duration-of-cleanliness claim.
Boeing multi-surface compatibility testing
Material compatibility
Boeing multi-surface compatibility testing assessed the Layer 1 surface chemistry across a range of materials and found it compatible with electronics, plastics, metals, and fabrics — directly relevant to mixed athletic environments and shared gear.
Source · Boeing multi-surface compatibility testing · reported as material compatibility (safe on materials), not as antimicrobial efficacy.
Our own measurement · Shield3
Field ATP readings
In March 2026, Shield3 took field ATP (RLU) readings on athletic gear (porous-foam knee and elbow pads), measuring surface bioburden before and after a single application under real conditions — one liberal spray, a 10-minute dwell, and overnight air-dry, with no wipe or rinse. ATP measures biological residue (in RLU) — a measure of cleanliness, not a pathogen-specific kill claim.
| Zone | Baseline RLU | After application | Bioburden change |
|---|
| Knee — outside (front) | 4,846 | 2,216 | 54.3% |
| Knee — inside (front) | 6,340 | 549 | 91.3% |
| Knee — inside (back) | 2,976 | 918 | 69.2% |
| Elbow — outside (funny-bone) | 1,334 | 642 | 51.9% |
| Elbow — outside (inner) | 4,765 | 2,790 | 41.4% |
| Elbow — inner (funny-bone) | 6,738 | 1,574 | 76.6% |
| Elbow — inner (inner) | 2,182 | 199 | 90.9% |
Average single-application reduction in measured surface bioburden: knee pad ~71.6%, elbow pad ~65.2%, with the largest reductions in the interior porous-foam zones.
Source · Shield3 field ATP readings · March 2026 · Hygiena enSURE Touch + UltraSnap. These are reductions in measured surface bioburden (RLU) under real conditions — not a pathogen-specific kill claim and not a duration-of-cleanliness claim. Field measurement is kept separate from the laboratory data above.
