Proof
Below is the evidence behind Shield3, drawn from independent laboratories, the manufacturer's testing program, and our own field measurement. Each result is shown with its source and reported on its own terms — laboratory efficacy and field bioburden are never merged.
Shield3's Layer 1 surface product (System 3 SDP) is the non-leaching silane quat chemistry manufactured by 2V Industries. The third-party testing below was conducted on that chemistry; Shield3 private-labels it.
5 evidence streams · each separately sourced
Independent lab–confirmed · Eagle Science-Based Solutions
Eagle Science-Based Solutions ran two complementary studies on the System 3 SDP chemistry in 2022: an efficacy study measuring reduction across a broad organism panel, and a dispersion study measuring how evenly the chemistry distributes when applied. Both are independently lab-confirmed.
The chemistry was evaluated against a 10-organism panel on three surface types — stainless steel, plexiglass, and vinyl — at a 10-minute dwell, under a protocol harmonizing EN 17272 and USP<1072>. Every organism on every surface met the study's acceptance criteria. Lab conclusion: the chemistry exhibits bactericidal, fungicidal, and sporicidal activity when used as directed.
| Measure | Result |
|---|---|
| Organism panel | 10 organisms (incl. Staphylococcus aureus / MRSA) |
| Surfaces tested | Stainless steel · plexiglass · vinyl |
| Dwell time | 10 minutes |
| Log reduction range | 4.5 to >6.0 across all organisms/surfaces |
| Outcome | All met acceptance criteria |
Source · Eagle Science-Based Solutions · Protocol PR-21047 · 2022
A dispersion study under EN 17272 measured how evenly the chemistry reaches a surface when applied. Against Staphylococcus aureus (ATCC 6538) on stainless steel at a 10-minute dwell, it achieved a ≥5-log reduction at every test zone, meeting the protocol's acceptance criteria.
| Zone | Log reduction |
|---|---|
| Zone 1 | 5.7 |
| Zone 2 | >6.0 |
| Zone 3 | >6.0 |
| Zone 4 | 5.7 |
| Challenge level | 7.3 × 10⁶ CFU / coupon |
Source · Eagle Science-Based Solutions · Protocol PR-21049 · testing 03/14/2022 · approved 03/25/2022
Reporting note: these are CFU-based microbiological challenge tests (not ATP), so log-reduction figures against named organisms are reported as the laboratory measured them.
Attributed · 2V Industries
GLP testing commissioned by the manufacturer evaluated System 3 SDP for bactericidal and virucidal efficacy at a 10-minute dwell, including activity against a coronavirus — reported as a 99.9% reduction in under three minutes.
Source · 2V Industries GLP testing · March 2021. Presented as an attributed third-party finding. Regulated marketing terms used in the manufacturer's own collateral (e.g., "hospital grade," "eradicates") are deliberately not carried onto this page.
Independent residual study
An independent residual study tracked surface bioburden by ATP (RLU) measurement over a three-week window under continuous heavy-touch conditions — surfaces in daily multi-user contact, with no re-cleaning during the study — using a Hygiena SystemSURE Plus ATP system.
Source · independent residual (Purifly) ATP study. Presented as a bioburden measurement and a description of the study design — not as a fixed "protects for three weeks" duration-of-cleanliness claim.
Boeing multi-surface compatibility testing
Boeing multi-surface compatibility testing assessed System 3 SDP across a range of materials and found it compatible with electronics, plastics, metals, and fabrics — directly relevant to mixed athletic environments and shared gear.
Source · Boeing multi-surface compatibility testing. Reported as material compatibility (safe on materials), not as antimicrobial efficacy.
Our own measurement · Shield3
In March 2026, Shield3 took field ATP (RLU) readings on athletic gear (porous-foam knee and elbow pads), measuring surface bioburden before and after a single application under real conditions — one liberal spray, a 10-minute dwell, and overnight air-dry, with no wipe or rinse. ATP measures biological residue (in RLU) — a measure of cleanliness, not a pathogen-specific kill claim.
| Zone | Baseline RLU | After application | Bioburden change |
|---|---|---|---|
| Knee — outside (front) | 4,846 | 2,216 | 54.3% |
| Knee — inside (front) | 6,340 | 549 | 91.3% |
| Knee — inside (back) | 2,976 | 918 | 69.2% |
| Elbow — outside (funny-bone) | 1,334 | 642 | 51.9% |
| Elbow — outside (inner) | 4,765 | 2,790 | 41.4% |
| Elbow — inner (funny-bone) | 6,738 | 1,574 | 76.6% |
| Elbow — inner (inner) | 2,182 | 199 | 90.9% |
Average single-application reduction in measured surface bioburden: knee pad ~71.6%, elbow pad ~65.2%, with the largest reductions in the interior porous-foam zones.
Source · Shield3 field ATP readings · March 2026 · Hygiena enSURE Touch + UltraSnap. These are reductions in measured surface bioburden (RLU) under real conditions — not a pathogen-specific kill claim and not a duration-of-cleanliness claim. Field measurement is kept separate from the laboratory data above.
How we handle evidence
How we report this
Laboratory efficacy and field ATP measure different things, in different settings. We present each on its own terms and never merge them. Third-party results are attributed to their source; our own field readings are labeled as ours.
Our claims standard
Efficacy data is presented as independent laboratory–confirmed. EPA registration is in progress with the manufacturer, and we will update this page as that status advances — we do not currently represent our products as EPA registered. We describe Shield3 as a persistent barrier layer that complements — does not replace — routine cleaning, we do not claim a fixed duration of protection, and we do not describe treated surfaces as "sterile."
Case Studies
Our case studies are produced from actual treatment data using a fixed reporting template. We don't invent results to fill the page — studies appear here once the data behind them is complete.
Mat and gear high-touch zones. Before/after field ATP under our standard protocol.
Full-facility surface and storage-air treatment.
High-touch surfaces and shared equipment.